Resveratrol is a stilbenoid with robust antioxidant exercise and several other useful properties for human well being. Plant extraction of resveratrol from pure sources is dear and non-sustainable, owing to the low amount of biomass and usually restricted availability. Biotechnological manufacturing of resveratrol can overcome these drawbacks. Right here, the heterologous resveratrol biosynthetic pathway (through phenylalanine) was expressed in a xylose-consuming Saccharomyces cerevisiae pressure. We additional elucidated the roles of the pentose phosphate pathway and dietary supplementation in resveratrol titres. By simultaneous fermentation of glucose and xylose, a 1.31-fold improve in resveratrol titre was noticed when put next with glucose-only cultivation on the identical carbon molarity, reaching a titre of 388 mg L−1. The recombinant pressure was capable of eat all sugars current in wine wastes, together with non-naturally metabolised sugars like xylose. This allowed the valorisation of various winery residues, akin to wine lees, grape should and hemicellulosic hydrolysate from vine pruning, reaching titres between 167.1 and 282.7 mg L−1 of resveratrol. The potential of environmental-friendly biotechnological processes over typical processes like plant extraction utilizing hazardous and polluting solvents is emphasised. That is the primary report on using renewable carbon sources for resveratrol manufacturing from xylose and using vineyard by-products as a substrate to provide this stilbenoid. The expanded multi-sugar utilisation capability of this yeast is effective in a biorefinery context and acquiring high-value merchandise akin to resveratrol is crucial to rising course of feasibility following a round economic system idea.