Biocatalytical upgrading of facet streams from agricultural biomass into multifunctional supplies constitutes a really enticing possibility to extend the circularity of meals and materials methods. We suggest the design of radical scavenging hydrogels with mechanical integrity and protecting results in opposition to reactive oxygen species by enzymatic crosslinking of arabinoxylans (AX) with excessive ferulic acid content material extracted from corn fibre utilizing subcritical water. We have now in contrast the affect of two enzymatic methods, laccase/O2 and peroxidase/H2O2, on the biochemical construction, multiscale meeting, physicochemical properties, and radical scavenging exercise of the polysaccharide hydrogels. Peroxidase crosslinking ends in immediate hydrogel formation, whereas laccase exhibits slower crosslinking kinetics, leading to a extra elastic gel community. Characterization by measurement exclusion chromatography, small angle X-ray scattering, and microscopy revealed structural variations within the community group of the hydrogels produced by the 2 enzymes. Laccase crosslinking results in smaller polymeric aggregates, selling their progressive group in community clusters that impression the general ultrastructure. Conversely, the quick crosslinking induced by peroxidase ends in greater porosity and types bigger and doubtlessly extra heterogeneous aggregates, which appear to hinder their subsequent affiliation in clusters. Each AX hydrogels exhibit enough biocompatibility and protecting results in opposition to in vitro mobile oxidative stress in comparison with an alginate reference. This constitutes a proof of idea of the potential utility of radical scavenging hydrogels from agricultural facet streams for biomedical and dietary purposes in wound therapeutic, mobile restore and focused supply.
