HomeMicrobiologyFungal β-glucan-facilitated cross-feeding actions between Bacteroides and Bifidobacterium species

Fungal β-glucan-facilitated cross-feeding actions between Bacteroides and Bifidobacterium species

Screening Bacteroides species for β-glucan metabolism

Sure members of the Bacteroides genus have been established as generalist fermenters of blended linkage (barley) and yeast β-glucan13,34,35,36,37. The genomes of those Bacteroides species embody varied PULs (right here known as glucan utilization loci or GULs) to degrade this advanced glycan, using encoded glycoside hydrolases for this metabolic course of. To evaluate the power of Bacteroides species to make use of fungal-derived β-glucan, we extracted this polysaccharide from mycoprotein of Fusarium venenatum (see “Supplies and strategies”) and screened the 23 most outstanding, gut-associated Bacteroidetes species for his or her skill to develop on this glycan in addition to on β-glucan derived from yeast (Desk S1). Below the (anaerobic) situations used, Ba. xylanisolvens, Ba. intestinalis, Bacova, Ba. fragilis, Ba. finegoldii, Ba. vulgatus, Ba. uniformis, BT (partial development), Dysgonomonas gadei, D. mossii and two strains of Baccell (Baccell WH2 and Baccell DSMZ) have been proven to metabolise β-glucan derived from mycoprotein. As well as, Ba. vulgatus, Ba. uniformis, Bacova, BT, Dysgonomonas gadei, D. mossii and each Baccell strains have been capable of utilise yeast β-glucan. Bacova has beforehand been proven to develop in barley-derived β-glucan as nicely34,35, exhibiting this species’ broad skill to ferment β-glucans from totally different sources and chemical buildings (Fig. 1A). Determine 1B reveals the expansion profile of Baccell WH2, Baccell DSMZ, BT and Bacova on β-glucan from mycoprotein (Fig. 1B). Along with these development experiments, we assessed development of varied Bifidobacterium strains on mycoprotein-derived β-glucan, clearly exhibiting a scarcity of skill by these strains to make use of this advanced carbon supply (Fig. S1A).

GUL regulation in Bacteroides and structure

To additional characterise development of chosen Bacteroides strains on explicit β-glucans, we carried out proteomics evaluation on Baccell WH2 when grown on glucose (performing as reference) or mycoprotein-derived fungal β-glucan as carbon sources. We in contrast the generated proteome information of this bacterium when grown on both of those carbon sources to determine proteins that exhibit elevated expression when the pressure is grown on the advanced polymer. As proven in Fig. 1C, this evaluation revealed that each one proteins encoded by two assigned GULs (named right here as GUL-1 and GUL-2 and representing locus tags BcellWH2_01929-BcellWH2_01932 and BcellWH2_02537-BcellWH2_02542, respectively, see Fig. 1D) exhibit elevated expression when Baccell WH2 was grown on mycoprotein-derived fungal β-glucan metabolism (when in comparison with development on glucose). GUL-1 was predicted to encode two GH3 enzymes (BcellWH2_01926 and BcellWH2_01927) and a GH157 member (BcellWH2_01931), whereas the proteins encoded by locus tags BcellWH2_01928 and BcellWH2_01929 characterize the SusC/D-like pair predicted to be concerned in (polysaccharide) substrate binding and recognition on the bacterial cell floor (Fig. 1D). Moreover, BcellWH2_01932 is predicted to characterize the Hybrid Two Element System (HTCS) sensing system controlling transcriptional regulation of GUL-1. GUL-2 encodes a GH30_3 (BcellWH2_02537, a predicted endo-β-1,6-glucanase in line with the CAZY database), a GH2 (BcellWH2_02541) and a protein with out recognized operate (BcellWH2_02538). Along with these proteins, BcellWH2_02539 and BcellWH2_02540 characterize the expected the SusC/D pair in GUL2 (Fig. 1D). To substantiate the proteomics information, we grew Baccell WH2 to mid-exponential and carried out RT-qPCR on chosen SusC/D pairs recognized within the above described GULs (Fig. S1B). As well as, we additionally used this strategy with BT and Bacova on the SusC/D pairs recognized primarily based on differential expression patterns when these two strains had been grown on beta-glucan from pustulan17,34,35 (Fig. S1B). Since we noticed differential expression primarily based on RT-qPCR, plainly their corresponding GULs, that are considerably totally different from that of Baccell WH2 by way of their genetic construction and content material, are additionally chargeable for development on fungal β-glucan. Determine 1D and S1C present the GUL structure of BT, Bacova and Baccell WH2, and predicted features pertaining to GUL regulation, β-glucan degradation and related oligosaccharide consumption. BT and Baccell WH2 make use of a special GUL structure for fungal β-glucan utilization (Fig. 1D), when in comparison with Bacova, which primarily based on its expression profile seems to make use of the identical GUL for fungal and barley β-glucan Figs. S1B and S1C.

As acknowledged above, BT, Baccell WH2 and Bacova can metabolise fungal β-glucan. Based mostly on qPCR carried out on BT rising in a medium containing both fungal β-glucan or pustulan, this bacterium seems to make use of the identical GUL for both of those two carbon sources17 (BT3309-BT3314, Fig. 1D and S1B). In line with qPCR information obtained when Bacova is rising in a medium supplemented with both barley or fungal β-glucan (Fig. S1B), the bacterium was proven to make use of the identical GUL for both of those carbon sources, which contrasts with what we found for Baccell WH2, as this bacterium seems to make use of distinct GULs to deconstruct both fungal or barley β-glucan (Fig. 1D and S1C)49.

Dissecting the enzymes encoded by GUL-1 and GUL-2 that degrade fungal β-glucan

Following our statement that sure proteins encoded by, and their corresponding genes current in, GUL-1 and GUL-2 of Baccell WH2 elicit elevated expression when rising on fungal β-glucan, we needed to substantiate their involvement on this metabolic course of. For this goal, we recombinantly expressed the enzymes representing the putative GH157, GH30_3 and GH3 (Baccell WH2_01926) actions from GUL1/GUL2 to completely dissect the mechanism of degradation of this advanced dietary carbon supply. For this goal, we incubated the expressed proteins with fungal β-glucan and revealed attainable degradation merchandise via HPLC chromatography. Extra particularly, Fig. 2A reveals the HPLC chromatograms of BcellWH2_01931 and BcellWH2_02537 (representing predicted GH157 and GH30_3 actions, respectively) performing on fungal β-glucan.

Fig. 2: HPLC evaluation of the enzymatic reactions of glycoside hydrolases in Baccell WH2 and BT on fungal β-glucan.
figure 2

All HPLC experiments have been produced in 3 totally different impartial replicates (n = 3). A Time course of BcellWH2_01931 (GH157) with mycoprotein β-glucan. B Time course of BcellWH2_01931 (GH157) with linear β-1,3-glucan. C Time course of BcellWH2_02537 (GH30_3) on mycoprotein β-glucan. D Time course of BcellWH2_02537 (GH30_3) with pustulan. E Time course of BcellWH2_01931 (GH157) and BcellWH2_02537 (GH30_3) collectively on linear β-1,3-glucan. F HPLC of BT3312 (GH30_3) on mycoprotein β-glucan. G HPLC of BT3314 (GH3) on mycoprotein β-glucan.

The info introduced in Fig. 2A and 2B confirmed that BcellWH2_01931 acts on fungal β-glucan (Fig. 2A) and the linear β-1,3-glucan from Euglena glacialis (Fig. 2B) initially releasing penta- and trisaccharides, that are transformed to disaccharides and glucose upon longer incubation (16 h). Nonetheless, we didn’t discover any exercise when the enzyme was incubated with pustulan. Desk 1 lists the kinetic parameters (okaycat/Okm) of this protein performing on both substrate.

Desk 1 Kinetic parameters of GHs in Baccell WH2, BT and Bi. breve UCC2003 performing totally different β-glucan substrates and gluco-oligosaccharides.

To validate the prediction that these enzymes are concerned in fungal β-glucan metabolism, we carried out protein location evaluation by LipoP50, indicating that BcellWH2_01931 (GH157) and BcellWH2_02537 (GH30_3) are situated on the cell floor of Baccell WH2. Moreover, in line with the CAZY database, the GH157 enzyme was anticipated to behave as an endo-β-1,3-glucanase, whereas the GH30_3 enzyme was anticipated to own endo-β-1,6-glucanase exercise as has been reported for different members of this CAZY household17. Like GH157, recombinantly expressed and purified GH30_3 was incubated with fungal β-glucan, linear β-1,3-glucan and pustulan (linear β-1,6-glucan), adopted by HPLC evaluation. Determine 2C and 2D present the related chromatography outcomes for this incubation experiment, confirming the β-1,6-glucanase exercise with fungal β-glucan (Fig. 2C) and pustulan (Fig. 2D). As well as, GH30_3 didn’t present any exercise towards linear β-1,3-glucan. Desk 1 shows the catalytic parameters of this protein as measured by DNSA assays with fungal β-glucan and pustulan. BcellWH2_02537 confirmed exercise parameters for β-glucan from Fusarium venenatum which can be like these obtained for pustulan (okaycat/Okm of 2512 ± 28 and 1968 ± 17 mg ml−1 min−1) suggesting that the enzyme doesn’t require interactions with the β-(1,3)-glucan spine. As well as, BcellWH2_02537 was proven to solely exhibit exercise on oligosaccharides bigger than β-(1,6)-glucotriose indicating that, as in BT3312, the enzyme has 3 subsites within the energetic website (nomenclature established by Davies et al.)51.

Lastly, to completely perceive how mycoprotein-derived β-glucan is metabolised by Baccell WH2, we recombinantly expressed the expected GH3 and GH2 enzymes (as specified by BcellWH2_01926 and BcellWH2_02541, respectively), exhibiting that each are capable of act on the totally different oligosaccharides produced by the motion of the GH157 and GH30_3 enzymes. BcellWH2_01926 (GH3) was capable of degrade β-1,6-glucooligosaccharides launched by GH30_3, whereas the GH2 enzyme (BcellWH2_02541) was proven to behave on β-1,3-glucooligosaccharides (glucobiose and glucotriose), in each instances releasing glucose as the ultimate product, which confirms the exo-acting mechanism for these enzymes (BcellWH2_01926 and BcellWH2_02541). Desk 1 additionally shows the catalytic parameters of those two exo-glucosidases, which highlights that the exercise of the GH2 enzyme is comparable for β-1,3-glucobiose and β-1,3-glucotriose, suggesting 2 subsites within the energetic website.

Within the case of BT, the GUL structure is easier than that noticed for Baccell WH2 (Fig. 1D). For the previous bacterium, solely the GH30_3 (BT3312) and a GH3 (BT3314) have beforehand been described to be energetic on pustulan β-glucan17. To evaluate the catalytic exercise of BT3312 on β-glucan from Fusarium venenatum, we carried out enzymatic assays with this polysaccharide acquiring exercise ranges which can be much like these of BcellWH2_02537 (2874 ± 35 mg ml−1 min−1 for BT3312 and 2512 ± 28 mg ml−1 min−1 for BcellWH2_02537) (Desk 1). The BT3312 enzyme was proven to be energetic on fungal β-glucan aspect chains, thereby releasing explicit β-1,6-oligosaccharides, which in flip could be hydrolysed by BT3314 as an exo-glucosidase to launch glucose (Fig. 2F for BT3312, and Fig. 2G for BT3314). In the identical manner as Baccell WH2 enzymes, Desk 1 reveals the catalytic parameters for BT3312 and BT3314 on these substrates.

Structural modelling of GH157 and GH30_3 from Baccell WH2

To dissect the interactions of the Baccell WH2-encoded GH157 and GH30_3 enzymes with fungal β-glucan, we tried to acquire crystals of the Baccell WH2 proteins. Sadly, regardless of screening a number of situations we have been unable to acquire appropriate crystals. As an alternative, we obtained the construction of the GH30_3 protein by comparability to the crystal construction solved for BT3312 utilizing the Phyre2 algorithm as a search platform17,52. Fig. S1D reveals that the expected construction is an (β/a)8 barrel with the conserved retaining mechanism the place two glutamic acids act as nucleophile (E339 and E347 for BT3312 and BcellWH2_02537, respectively) and acid/base (E238 and E247 for BT3312 and BcellWH2_02537, respectively). As indicated on this Determine, each proteins present a excessive stage of sequence similarity (58.33% equivalent) and exhibit the identical exercise profile (Desk 1). This means that the GH30_3 from Baccell WH2 comprises solely three main subsites in an analogous method to what has been described for BT331217. Amino acids concerned within the binding and catalysis are conserved in each proteins (Fig. S1E).

Oligosaccharides launched into the cultivation medium

To research if Baccell WH2 and BT launch oligosaccharides into their cultivation medium when grown on fungal β-glucan, thereby maybe permitting development of different micro organism by way of cross-feeding actions, we obtained cell free development medium following cultivation of those strains to the mid-exponential and stationary section (Fig. 3). The presence of oligosaccharides launched into the media by Baccell WH2 or BT was then assessed by HPLC (Fig. 3A, B). When these two bacterial species use mycoprotein as their sole carbon supply (Fig. 3A), they have been proven to launch oligosaccharides into the media, however these have been totally different in every case (Fig. 3B), which is in step with their totally different GUL structure and related enzymes, thus confirming the distinct degradative technique adopted by every of those two species.

Fig. 3: Characterization of oligosaccharides launched by Bacteroides when utilizing β-1,3-glucan.
figure 3

A HPLC chromatogram of the expansion media of Baccell WH2 on fungal β-glucan. B Identical as Panel A with BT. C Purified oligosaccharide from Baccell WH2 after Gel Filtration (GF) column. D Purified oligosaccharide from BT after GF column. E LC/MS of Baccell WH2 supernatant grown on fungal β-glucan. F LC/MS of BT supernatant grown on fungal β-glucan. All HPLC and LC/MS experiments have been carried out in 3 totally different impartial replicates (n = 3).

To research the character of those oligosaccharides, we carried out LC/MS to find out their mass. Determine 3C reveals the HPLC profile of the purified important oligosaccharide launched by Baccell WH2 and Fig. 3E the related mass spectrum of this oligosaccharide confirming that this bacterium predominantly releases a heptasaccharide. These outcomes are in concordance with the in vitro enzymatic digestion of fungal β-glucan as a result of once we incubated this fungal carbon supply with GH30_3 and GH157 collectively, the merchandise generated by each enzymes have been glucose, 1,6-β-glucobiose 1,3-β-glucotetraose and the heptasaccharide current within the development medium generated by Baccell WH2 (Fig. 2E). Determine 3F shows the mass spectra of the principle oligosaccharides launched by BT within the development medium, confirming the presence of a disaccharide as the principle product, akin to 1,6-β-glucobiose as was indicated within the HPLC chromatogram (Fig. 3D).

Cross-feeding Bacteroides/Bifidobacterium/Lactiplantibacillus

After we confirmed the discharge of oligosaccharides into the expansion medium by Bacteroides when grown on mycoprotein β-glucan, we hypothesised that this phenomenon would enable different intestine commensals to cross-feed on such launched oligosaccharides.

Certainly, Fig. 4 reveals that Bifidobacterium strains are capable of develop when co-cultivated with Bacteroides on fungal β-glucan. We screened the in a single day supernatant from Baccell WH2 and BT grown in β-glucan with a number of out there Bifidobacterium and Lactobacillus strains exhibiting that, within the case of Baccell WH2 supernatant, Bi. breve UCC2003, Bi. longum subsp. longum NCIMB 8809 and Lb. plantarum WCFS1 are unable to make use of intact β-glucan as a carbon supply, but that they’ll utilise the heptasaccharide launched by Baccell WH2 (Fig. 4A). We confirmed the power of those strains to make use of the heptasaccharide testing the expansion media earlier than and after the Bi. breve UCC2003, Bi. longum subsp. longum NCIMB 8809 and Lb. plantarum WCFS1 development. Determine 4B confirms using the heptasaccharide by Bi. longum subsp. longum NCIMB 8809, with Bi. breve UCC2003 and Lb. plantarum WCFS1 exhibiting a extra modest skill to make use of the launched oligosaccharide too. After 24 h fermentation, Bi. longum subsp. longum NCIMB 8809 reached a ultimate optical density of 0.8 when utilizing the supernatant from Baccell WH2. Bi. breve and Lb. plantarum have been ready to make use of these oligosaccharides however to a lesser diploma, reaching a ultimate optical density of 0.6.

Fig. 4: Consumption of β-glucan oligosaccharides launched by Bacteroides into the expansion media by Bifidobacterium species.
figure 4

A Development of Bifidobacterium with fungal β-glucan supernatant from Baccell WH2. B HPLC evaluation of supernatants earlier than and after development of Bifidobacterium longum subsp. longum on supernantants from Baccell WH2. C Development of Bifidobacterium with fungal β-glucan supernatant from BT. D HPLC evaluation of supernatants earlier than and after development of Bifidobacterium longum subspecie longum on cell-free supernatant of BT grown on fungal β-glucan. E HPLC evaluation of supernatants earlier than and after development of Bi breve UCC2003 and L. plantarum on cell-free supernatants of BT grown on fungal β-glucan. All growths and HPLC experiments have been produced in 3 totally different impartial replicates (n = 3).

When the supernatant of BT was used as carbon supply for the screening of Bi. breve UCC2003, Bi. longum subsp. longum NCIMB 8809 and Lb. plantarum WCFS1, the disaccharide which is in any other case accumulating within the medium is not current, indicating that this β-1,6-glucobiose is utilized by the bifidobacterial and Lactiplantibacillus strains to maintain their development (Fig. 4C, D). Evaluation of the expansion medium by HPLC was additionally carried out for Bi. breve UCC2003 and Lb. plantarum WCFS1 confirming their skill to make use of the β-1,6-glucobiose as carbon supply (Fig. 4E).

To substantiate this newly found cross-feeding interplay between Bacteroides and Bifidobacterium species, we carried out cross-feeding experiments at totally different time factors the place we checked a co-culture of each species monitoring the colony forming models and 16 S rRNA-based qPCR quantification of every species throughout development (Figs. 5, 6). On this co-culture experiment, Baccell WH2 allowed Bi. longum subsp. longum NCIMB 8809 and Bi. breve UCC2003 to develop when each strains are cultivated with the intact fungal β-glucan as is clear from viable depend assessments (Fig. 5A for Bi. longum subsp. longum and 5 C for Bi. breve) and proportion of each micro organism within the co-culture (Fig. 5B for Bi. longum subsp. longum and 5D for Bi. breve). The statement of Bifidobacterium development when in co-culture with Baccell WH2 within the presence of β-glucan agreed with the monoculture fermentation findings, when cell-free supernatant was used as carbon supply, as displayed in Fig. 4.

Fig. 5: Cross-feeding experiments between Baccell WH2 and Bifidobacterium and Lactiplantibacillus spp.
figure 5

A Colony forming models of Baccell WH2 + Bi. longum subsp. longum. B Proportion of Baccell WH2 + Bi. longum subsp. longum. C Colony forming models of Baccell WH2 + Bi. Breve UCC2003. D Proportion of Baccell WH2 + Bi. breve UCC2003. All cross-feeding experiments have been produced in 3 totally different impartial replicates (n = 3).

Fig. 6: Cross-feeding experiments between BT and Bifidobacterium and Lactiplantibacillus spp.
figure 6

A Colony forming models of BT + Bi. breve UCC2003. B Proportion of BT + Bi. breve UCC2003. C Colony forming models of BT + Bi. longum subsp. longum. D Proportion of BT + Bi. longum subsp. longum. All cross-feeding experiments have been produced in 3 totally different impartial replicates (n = 3).

Equally, BT allowed development of Bi. breve UCC2003 and Bi. longum subsp. longum NCIMB 8809 in co-culture as could be noticed in Figs. 6A and 6C (colony forming models) and 6B and 6D (proportion), respectively. Once more, it confirmed the power of Bacteroides to permit for particular cross-feeding networks with Bifidobacterium strains within the intestine when the previous micro organism develop on dietary fungal β-glucan.

Lastly, to develop this research with different commensal members of the human intestine microbiota, we carried out the co-culture experiment of Baccell WH2 and BT as major and Lactiplantibacillus plantarum WCFS1 as a secondary degrader to substantiate the power of Baccel WH2 and BT to permit development of this commensal. Figs. S2A and S2B for Baccell WH2 and Figs. S2C and S2D for BT confirmed this skill in co-culture too.

Means of Bi. breve UCC2003 to make the most of β-1,6-glucobiose

As acknowledged above, Bi. breve UCC2003 can use glucobiose launched by Baccell WH2 when grown on β-glucan. We carried out a bioinformatic evaluation on the genome of the previous bacterium to determine glycoside hydrolases that might enable Bifidobacterium to utilise this oligosaccharide. We recognized a GH1 (Bbr_0109) which had beforehand been proven to behave on cellobiose as substrate53. We hypothesised that this protein would act on gluco-oligosaccharides with totally different linkages as substrates. To show our speculation, we recombinantly expressed the protein and carried out enzymatic assays with β-1,4, β-1,3 and β-1,6-glucobiose as substrates of the enzyme. Bbr_0109 was energetic on β-1,4 and β-1,6-glucobiose as we anticipated, and this exercise is proven by HPLC in Figs. S3A and S3B, respectively. As well as, we have been capable of characterise this exercise and the kinetic parameters are calculated in Desk 1. Bioinformatics evaluation of the Bi. longum subsp. longum genome didn’t reveal any homolog of Bbr_0109, or different candidate enzymes chargeable for its skill to cross-feed on β-glucan-derived oligosaccharides and additional work is due to this fact required to unravel the metabolic pathway chargeable for this exercise.

Metabolites launched by Bacteroides/Bifidobacterium co-cultivation on β-glucan

After we confirmed the power of Bacteroides to particularly enable Bifidobacterium development when the previous bacterium is cultivated on β-glucan, we carried out a metabolomics evaluation of the tradition media to evaluate brief chain fatty acid (SCFA)/lactate/succinate manufacturing by Bacteroides and Bifidobacterium in mono- and co-cultures. Desk 2 lists the detected ranges of acetate, butyrate, propionate, and lactate by these fermentations. Baccell WH2 was proven to provide succinate (78 mM) and acetate (12 mM) as important SCFAs produced in monoculture. As management, Bi. longum subsp. longum NCIMB 8809 failed to provide any important quantities of SCFAs due to its incapability to make use of the intact mycoprotein β-glucan. In distinction, in co-culture, acetate was the upper concentrated SCFA (115 mM) adopted by succinate (99 mM) as consequence of the subsp. longum NCIMB 8809 development. Lastly, formate (16 mM) and lactate (21 mM) have been additionally produced in co-culture due to the power of Bifidobacterium to provide these metabolites.

Desk 2 Millimolar (mM) focus of the metabolites generated within the cell-free supernatant of MM + 1% β-glucan, following 24 h incubation with Baccell WH2 and B. breve UCC2003.


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